ZORBAXQuick guide to reverse phase bonding
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Current ZORBAX RP-HPLC chromatographic column |
Usage and application suggestions |
Eclipse Plus |
The chromatographic column developed by the method; Acidic, alkaline, and neutral compounds can be reliably separated at pH 2-9 and have a long lifespan; Excellent peak shape for the separation of alkaline compounds; 1.8, 3.5, and 5 µ m chromatography columns have high separation and column efficiency; Through rigorous QA/QC testing, ensuring long-term reproducibility |
Eclipse XDB |
There are four options to choose from, allowing for flexible method development; High performance within a wide pH range (PH2-9); Has excellent peak shapes for acidic, alkaline, and neutral compounds; Ultra tight bonding and double sealed ends extend the lifespan; Use 1.8 and 3.5 µ m chromatography columns for rapid, ultra high speed, and high resolution separation; There are capillary columns to prepare columns to choose from |
StableBond(SB) |
Alkaline, acidic, and neutral compounds; Especially stable at low pH values; Using high temperature (up to 90 ℃ for C18 and up to 80 ℃ for C8, C3, phenyl, cyano, and Aq) and low pH as selective means to increase selectivity; There are multiple bonding phases available for different selectivity options (C18, C8, C3, cyano, phenyl, etc.) Aq); LC/MS mobile phase containing formic acid, acetic acid, or trifluoroacetic acid (TFA) can be used; Proteins and peptides can be separated using a mobile phase containing trifluoroacetic acid (TFA); Rapid separation using 1.8 and 3.5 µ m chromatography columns |
ZORBAX Rx |
The universal column for separating alkaline, acidic, and neutral compounds at low pH values has different selectivity compared to the SB column; Rx-C8 is the same as SB-C8S |
Bonus-RP |
Separate alkaline compounds using a mobile phase with high water content; A universal column for separating alkaline, acidic, and neutral compounds at medium or low pH; Especially stable at low pH; Separate peptides with different selectivity; Using a 3.5 µ m chromatographic column for rapid separation |
Extend-C18 |
Separate free forms of alkaline compounds above their pKa; Separate alkaline, acidic, and neutral compounds under high pH conditions; The maximum pH can reach 11.5; Using amine hydroxide as the mobile phase for LC/MS separation of small molecule compounds or peptides; Separate at high, medium, and low pH levels to alter selectivity; Using a 3.5 µ m chromatographic column for rapid separation |
earlyZORBAXchromatographic column |
Usage and application suggestions |
ZORBAX |
The universal separation of alkaline, acidic, and neutral compounds at low pH is different from the selectivity of SB chromatography columns; Under relatively neutral pH conditions, the number of active silanol groups separated in 'mixed mode' exceeds that of SB column |
ZORBAX ODS Classic (unsealed) |
A universal column for separating alkaline, acidic, and neutral compounds at medium to low pH values, with different selectivity compared to SB or XDB chromatography columns |
