Cross flow chromatography is an ideal choice for separating and purifying biomolecules, widely used in ion exchange chromatography, affinity chromatography, and hydrophobic chromatography. The medium of cross flow chromatography has a unique macroporous structure that penetrates through microspheres, which can significantly reduce the mass transfer barriers within the pores of biomolecules. The safety and reliability of cross flow chromatography technology have been demonstrated in the United StatesFDAVerified in the production of approved biological therapy products.
● large aperture
300-600nmPore size makes it easier for biomolecules to diffuse and transfer mass
● Multiple particle sizes to choose from
45-90Micro particle size to meet different purification requirements
● Rigid microspheres
Maximum pressure tolerance up to870psi (60 bar)Excellent mechanical performance
● 柔性触手
High recovery rate, high target purity, good binding ability, and strong capture capability.
● Strict in-situ cleaning conditions(CIP)
0.5-1M NaOHofCIPCondition, able to tolerate organic solvents and high salt solvents
● Excellent chemical stability
VirCap ® AFThe medium is based on rigidityPS-DVBMicrospheres are hydrophilic porous polymer fillers with specific adsorption function, and the heparin like groups on the surface can interact with certain viruses with capsule membranes. It can be widely used for the purification of certain viral vaccines.
|
matrix |
hydrophilicityPS/DVBmicrosphere |
|
average particle size |
70um |
|
functional group |
Heparin like substance |
|
Dynamic load capacity |
50mg lysozyme/ml |
|
flow velocity |
1000cm/h(20℃,Use process buffer with the same viscosity as water,Under pressure<3bar(0.3MPa),The height of the column bed is20cm) |
|
Column bed height |
20-40cm |
|
pHstability |
1-14 |
|
temperature |
4-30℃ |
|
chemical stability |
All commonly used buffer solutions,2M NaOH |
|
store |
2-8℃20% EtOH |
