Protein MarkerThe high-throughput protein purification system is suitable for protein purification in high-throughput proteomics laboratories, solving the bottleneck of current protein purification and allowing researchers to set or run standard medium pressure chromatography experiments, which can reach up to24A separate chemical column, each with its own buffer system (equilibrium buffer)AWash buffer solutionB，strip bufferAnd water).Protein Makercombination24A precision syringe pump, each with8A valve head, a new pump system, and a high-resolution bucket rack can accurately transmit.

Protein MarkerThe high-throughput protein purification system mainly includes pump racks, tube racks, and control software. The pump frame includes24A different syringe pump（1~50mLCapacity), each connected with8Each valve is connected to a different solution when used5mLThe flow rate during injection can be adjusted to0.25~20mL/min.

Up to24A standard pre filled column（1~10mL）Can be installed on the valve, and there are20A location for placingSBSPlates or blocks can be used for loading samples or specialized washing buffer solutions. Clicking on the user interface allows operators to select different columns or change parameters for automated operations, as well as perform manual suction and dispensing operations from start to finish,Protein Makerfunction24A different one1mLThe column takes approximately one hour, including individual adjustments to the reaction gradient (input at the beginning)24-10mLProtein samples).

Suitable for protein purification in high-throughput proteomics laboratories

1. Parallel protein chromatography

2. 24A parallel purification

–Production Mode（24A different protein sample,1Types of chromatographic resins

–Scouting Mode2(4A different chromatographic resin, a protein sample)

–Complex Purification Mode(Two dimensional chromatography)

3. Can run24A chromatographic column（0.1~10mL）

4. Fully automatic operation

5. Wide sample size -0.5mLreach250mLSample size

Protein MakerSoftware and user interface

1. Select operation method and number of loops

2. Set the traffic for each operation

3. Choose the number of cycles for balancing and washing

4. Set the volume of each board and the composition of the detergent

5. Fully automated control of the position of every pump, valve, and pipe rack

application

•250mLPurification of antibodies in the initial volume（i.e.proteinAAffinity chromatography)

Preparation of samples and discovery of biomarkers (plasma and cerebrospinal fluid) for mass spectrometry detection using immune loss resin

•(surface)Parallel expression and purification detection of induced protein construction( Ni-chelate chromatography)

•24Protein samples can be processed in parallel,The protein production can be sufficient for a large number of analytical experiments