TIRF、 Focusing togetherFRET、 Photoactivation and microinjection techniques have helped scientists overcome many difficulties in imaging live cells. The core of all technology is Ti, and with this powerful new inverted microscope, you can use it on the Nikon CFI60 ® With the help of optical systems, the above technology can be easily used. The Ti series has three models, and the improved system speed, enhanced flexibility, and efficient multi-mode characteristics make Ti an ideal system for high-end research and live cell imaging.

Nikon's world leading optical designer has developed a unique external phase difference unit. By using this innovative system, the phase difference ring is integrated into the microscope body instead of the objective lens. Users do not need to use a dedicated phase difference objective lens to observe phase difference images and can obtain high-quality images through a high numerical aperture objective lens. In addition, using an objective lens without a phase difference ring can obtainFull brightness fluorescent image.

The optical path design of placing the phase difference ring originally placed in the phase difference objective lens on the external phase difference unit of the microscope body facilitates users to obtain high-resolution phase difference images using a high numerical aperture objective lens. There are four types of phase difference rings to choose from based on the objective lens used（Ti-E/U/S universal).

Use Nikon's high-performance objective lenses, includingThe 60x and 100x TIRF objectives have the world's highest numerical aperture of 1.49, and integrate a spherical aberration correction ring to obtain high-resolution phase difference images that cannot be compared to other standard phase difference objectives.

Obtained using the same objective lensDue to the absence of a phase difference loop, the "full brightness" fluorescence image suffers from light loss. In the same system, not only can phase difference observation be performed, but brighter "full brightness" fluorescence images, confocal images, and TIRF images can also be obtained.

By using an external phase difference unit, clear and high-resolution phase difference images can be obtained even with immersion lenses.

Due to the difference between the image andTIRF observation and DIC observation can use the same objective lens, and the obtained images can be used for high-precision data processing and image analysis, such as defining cell contours in TIRF images.

Having left port, right port, and bottom*The multi image port design of the port can connect one camera to each port. In addition, the expansion space design of the layered structure can add a rear port, which facilitates users to use double-layer fluorescent filter block boxes and multiple cameras for image acquisition. *Ti-E/B and Ti-U/B combination optional bottom port

The optional rear port design has expanded the image acquisition capability. Combined with the side port, two cameras can be used to capture dual channel images. For example, whenWhen there is an observation interval between fluorescent proteins in FRET (Foster Resonance Energy Transfer) and there is a significant difference in the intensity of CFP and YFP, high signal-to-noise ratio images can be obtained by adjusting the sensitivity of a single camera for comparison.

The layered structure adopted by Ti fully utilizes the advantages of infinite optical systems, and alsoPFS integrated into the objective lens converter. Two optional components other than PFS can be introduced into the optical path through a spacer block, and this system can simultaneously use laser tweezers, photoactivation units, and epifluorescence devices. Each layer's electric fluorescent filter block box can be individually controlled.

By introducingThe 870nm wavelength blocking device allows researchers to use near-infrared fluorescent dyes including Cy5.5. The optical properties in the ultraviolet to infrared range have been improved, and the number of available objective lenses has increased. Focus stability can be achieved in a wide range of applications, regardless of Ca in the ultraviolet range²⁺Concentration measurement is still using laser tweezers in the infrared range.

right96 well plate for fast three channel (dual channel fluorescence and phase difference) shooting, with a speed increase of more than 2 times.

Focus drift is the biggest obstacle in time series observation. Nikon'sThe PFS system corrects for focal drift that may occur during long-term observation and dosing. Even when using high magnification lenses or techniques such as TIRF, the focus can still be maintained. In addition, integrating PFS on the objective converter helps save space and does not limit the expandable layered structure of Ti. PFS adopts an efficient optical compensation system to perform real-time correction on the Z-axis plane. When PFS is not needed, it can be easily removed from the optical path.

Nikon's latest digital control hub significantly improves overall operational speed by reducing communication time between components and increasing the speed of various accessories.PC control optimizes the electric components of Ti, shortening the response time from action commands to movement, thereby implementing high-speed control of the entire system. By adding intelligent firmware, the overall operation time of electric components is significantly reduced, for example, the total time required for continuous image acquisition of three channels (dual channel fluorescence and phase difference) is greatly shortened, reducing phototoxicity to cells.

By synchronously controlling several electric components such as objective lens converters, fluorescent filter blocks, light gates, condenser converters, and stages, researchers can conduct multidimensional electric experiments. Faster attachment movement and image acquisition shorten overall exposure time, reduce corresponding phototoxicity, and help researchers obtain more meaningful data.

operation and/Or convert the objective lens, filter block XThe speed of the Y-stage and excitation/blocking filter has significantly increased, allowing researchers to focus on observation and image acquisition. The newly developed controller can record and replicate observation conditions, enabling the use of a mouse to control the stage. The entire microscope is like an extension of the researcher's eyes and hands.

Nikon's optimized optical technology provides multiple modes of observing specimens, presenting every detail of cells to researchers.

NomarskiDifferential interference（DIC）

The balance between high contrast and high resolution is crucial for observing subtle structures. Unique to NikonThe DIC system can obtain high-resolution images even at low magnification. The new DIC slider (dry) offers two options: high resolution and high contrast. The filter block type DIC analyzer can be placed inside an electric filter block box, significantly reducing the switching time between DIC observation and fluorescence observation.

It can be used for observing phase difference imagesCFI Plan Fluor ADH 100x (Oil)。 Compared with traditional phase contrast lenses, this objective reduces the halo of phase contrast images and enhances the contrast of the images.

Using highNA's spotlight can be used for dark field observation. It is possible to observe particles for a long time and avoid photobleaching.

HMC objective lens andThe combination of HMC spotlight components can obtain high contrast, halo free images similar to 3D, which can be applied to transparent samples cultured in plastic culture dishes.

CFI S Plan Fluor ELWD/ELWDPhase difference objective lens

The newly developed objective lens is capable of detecting near ultraviolet radiation（Light in the wavelength range from Ca2+to near-infrared has high transparency and improved color correction. High quality color free images can be obtained in various lighting modes.

Plan Apochromat 20xobjective lens

new-typeThe 20x objective has been added to Nikon's proprietary VC objective series, with axial chromatic aberration correction up to 405nm, making it an ideal objective for confocal observation and photoactivation technology.

All buttons and control converters used for electric operation are designed to be very user-friendly, allowing researchers to focus on their research without being affected by microscope operations.

Switching of fluorescent filter blocks, objective lens conversionZ-axis coarse/fine adjustment, PFS on/off control, and transmission illumination on/off control can all be quickly switched through the buttons located on the microscope body.

High speed electric vehicles can be controlled through a joystick or human-machine controllerXY stage and Z-axis.

Microscope status including objective information andThe on/off status of PFS will be displayed on the VFD screen. Adjust.

PFScompensation function

The compensation function of PFS is easy to control, and can be switched with just one button/Fine tuning.

The microscope can be operated and its current status can be confirmed through the remote control panel. Additionally, the observation conditions can be automatically switched through preset buttons. With just one button, you can switch from phase difference to fluorescence observation.

Tilting the front of the microscope body slightly backwards shortened the distance between the operator's eye points and the specimen by about40mm， Enhanced operability.