Sharp tools make good work. To conduct research on a specific characteristic of the root system, it is necessary to find suitable research methods and equipment. This plan provides a comprehensive solution for indoor and outdoor root science research.

LK-1200Desktop root analyzer

LK-1200The desktop root analyzer is designed for rotational imaging after root washing, and can continuously perform two-dimensional imaging analysis on plant roots in large quantities. It can measure hundreds of plant samples per day; Continuous rotational imaging of root systems from different angles can be performed as needed.

By combining professional control software and image analysis, the length, diameter, area, biomass, and root topology of the entire plant root system can be measured; In addition, the instrument is equipped with a barcode scanner, which can automatically generate image names of root samples, saving measurement time; This instrument can be widely used in research and teaching related majors such as agriculture, biology, ecology, cultivation, and horticulture.

Indoor Test Series

Field experiment series

Product Features

• 2044Ten thousand pixel high-definition imaging module;

• Equipped with a barcode scanner, it can quickly name root images;

• The root system can automatically360Rotation can capture two-dimensional images at any angle;

• The imaging system can automatically adjust the frame on the track according to the size of the root system;

• Powerful root image analysis software that can measure20Multiple root system indicators;

• Three dimensional synthesis and analysis software can be selected in the later stage for three-dimensional root image analysis.

LK-1250Plant Root Box Imaging System

The LK-1250 plant root box imaging system is designed for long-term continuous non-destructive measurement of plant roots under controlled conditions. Plants are planted in transparent root boxes on the bottom, and the root boxes are tilted and placed on fixed angle brackets. The plant roots are mainly distributed on the transparent root box panel due to gravity.

Combined with a professional root box imaging system, continuous tracking and measurement of root system changes throughout the entire growth cycle of plants can be achieved. Image analysis software can measure root system indicators such as length, diameter, area, biomass, and topological structure; At the same time, professional porous soil pore water micro probes can be used on the back of the root box to extract rhizosphere soil solutions and study the interaction between plant roots and soil.

main features

• Paired with a dedicated root box to meet the long-term non-destructive imaging measurement needs of root systems;

• Imported from Germany2044Ten thousand pixel high-definition imaging module;

• Rolling guide rail design and scanning code to name root images can save a lot of experimental time;

• The material (transparent or opaque) and specifications of the root box can be customized according to the user's needs;

• Multiple root image analysis software can be selected for measurement20Multiple root system indicators.

practical application

To investigate the effects of soil nutrients on the morphology and development of rice roots, experiments were conducted in root boxes. The left side of the figure shows nutrient rich areas, while the right side shows nutrient deficient areas. The rice root system directly above tends to grow on the left side. utilizeLK-1250The root box imaging system monitors the dynamic development of roots in real time, and combines root image analysis software for visual analysis, as shown in the following figure.

MS-190 In Situ Plant Root System Analyzer

By using the classic micro root window technique, it is possible to observe and study the growth, death, and distribution (root architecture) of plant roots, mycorrhizae, and even root nodules in situ. The biggest advantage of this system is that it continuously monitors the changes in a single fine root from birth to death without interfering with the root growth process. It can also record the growth, development, and phenology characteristics of fine roots, root hairs, and even root nodules.

main features

• 360Degree blind spot imaging; Resolution can reach2500dpi；

• Equipped with non-linear calibration function, it can eliminate the surface effect of micro root canals;

• Imaging speed is less than1Seconds, no need for white balance, can efficiently capture images;

• Image capture and naming followICAPPlan to be compatible with various root analysis software;

• Equipped with classic“Smucker”Module, achieving precise positioning;

• The system can choose to configure professional root image analysis software;

Root700Root growth monitoring system

Root700The root growth monitoring system can obtain high-definition image information of plant roots in situ under natural conditions, assist in obtaining important parameters of plant roots through professional analysis software, and provide research data on underground root growth for researchers in plant root ecology, stress resistance, and other fields.

main features

• The angle offset correction function can adjust the angle formed between different levels of root systems, especially suitable for spatiotemporal comparative analysis of root systems;

• It has rich data chart style selection functions such as line chart, bar chart, scatter plot, area chart, etc;

• It can edit and manage information such as the time, location, naming, event recording, and annotations of the collected images,

• Capable of rotating and resolving imported images（DPI）Adjust attribute functions such as changing, resizing images, adjusting image contrast, and sharpening images;

• It can achieve mouse selection of specific areas, zooming in and out of local observations, counting selected areas, assisting in cropping contaminated areas, and using eraser function to correct statistical effects,

• The software comes with a calibration function to achieve semi-automatic size calibration,XYCalibrate and correct the length parameters of the object separately;

• The software is capable of analyzing color information of image objects, determining root survival rate, and outputting root diameter, length, and surface area marked with different colors.

Parameters

1. resolving power:1200dpi；

2. Scanning speed:5-15s；

3. Scanning angle:360°；

4. Measurement accuracy: Measurement accuracy: Length accuracy≤±1%Area accuracy<1%(High quality image quality) or≤3%(Standard image quality);

5. Measurement parameters: biomass parameters, single root parameters, directional trend parameters, mycorrhizal parameters, root topology parameters;

6. Transparent observation tube size: international standard7mmOuter diameter,64mmInner diameter and length can be customized;

7. Work environment:0 – 50℃Relative humidity0 – 100%RH(No condensation);

LK-1100Portable multispectral plant root analyzer

main features

• Highly portable design;

• mobile phoneappWireless control;

• Remote real-time transmission of root system images;

• Automatically concatenate the entire root system image;

• Configure automatic image background segmentation software;

• Automatically obtain the main indicators of root system.

TECHNICAL INDEX

1. Simultaneously obtaining5individualRGB+UV+NIRBand root image（405，540，590，660，940nm）；

2. 1100Ten thousand pixel camera;

3. The highest image resolution reaches4800dpi；

4. The imaging rate is less than5Seconds;

5. mobile phoneappReal time image preview;

Research difficulties

The limited image resolution provided by existing plant root analyzers not only hinders detailed analysis of rhizosphere morphology and structure (such as root hair length and mycorrhizal hyphal growth), but also limits the accuracy of visual analysis of roots. In addition, manual root tracing and soil background segmentation is time-consuming and labor-intensive, which limits in-depth research on plant roots.

The main manifestations are as follows:

1.Periodic image acquisition is required and remote operation is not possible, greatly increasing the workload;

2.Manual operation leads to the inability to continuously image and obtain real-time dynamics of roots;

3.During the imaging process, the image is blurred due to manual movement of the camera.

LK-1100As a result, it was born!

• Using multispectral technology to solve problems. The use of spectral reflectance for soil background segmentation reduces the time and error of manual root tracing; Multispectral analysis can effectively compare mycorrhizae, hyphae, as well as live and dead roots;

• Adopting wireless control and ultra portable design, convenient for field operation;

• Automatic image stitching and soil background segmentation have made it possible for fully automated analysis of root images; This will greatly reduce the workload of root researchers.

Long term field monitoring instruments

AS-18Fully automatic multispectral plant root analyzer

AS-18A fully automatic multispectral plant root analyzer is a measurement system for long-term positioning and monitoring of plant root growth dynamics. It is possible to observe and study the growth, death, and distribution (root architecture) of plant roots and mycorrhizae, as well as root nodules, in situ. Without interfering with the root growth process, continuous monitoring of the changes in a single fine root from birth to death can also record the growth, development, and phenological characteristics of fine roots, root hairs, and even root nodules.

Specification

1. Automatically repeat the acquisition of root and rhizosphere images;

2. Ultra high definitionRGBCamera, fixed focal length (dynamic resolution in)0.05-0.003125Supports timed shooting and high-definition video recording;

3. A replaceable lighting device with a strength of0-256Controllable level;

4. 360°Imaging with angular resolution0.36-0.0225°between;

5. The distance (vertical) and angle (rotation) between images can be modified through parameter programming;

6. The longest micro root canal2Meter, diameter7Centimeters, inner diameter6.4Centimeters, supporting vertical or certain angle installation;

7. Automatic correction of image distortion and automatic cropping;

8. The operation control center and motor are installed together on the top, sealed and waterproof;

9. pass through12Vperhaps24VDC power supply can also be provided by solar cells, buffer batteries, or AC-DC converters;

10. The maximum length of the root canal can be extended to3-5m.

System Characteristics

1.Software automatic control - can perform fully automatic continuous observation according to a predetermined program;

2.Root Image Automatic Analysis - Root Dynamic Tracking and Image Automatic Analysis Software.

Root system dynamic tracking

Image automatic analyzer

RhizonRoot zone soil pore water sampler

RhizonRoot zone soil pore water samplerSeparating rhizosphere soil from non rhizosphere soil and studying soil pore water, root system interactions, and other feasible methods can also be used to monitor root growth, metabolism, and depth distribution. Sampling analysis can also be conducted on defined root types (such as age class, root sequence) or soil regions (rhizosphere/non rhizosphere soil).

practical application

existRhizonboxUsed inMicroRhizonRoot zone soil pore water sampler for soil water extraction and utilizationRhizonIrrigation with rhizosphere soil irrigator.MicroRhizonThe rhizosphere soil pore water sampler is installed atRhizonboxIn the small holes of the soil on the upper wall, each oneMicroRhizonThe rhizosphere soil pore water sampler is connected toPVCSeparated sample vials in a vacuum box. By gravity, nutrient solution enters from a container located at a higher positionRhizonboxThe soil in the middle.

MicroRhizonRhizosphere pore water sampler

Used in conjunction with the root box

Extract pore water from rhizosphere soil or sediment using a simple, non-destructive, and reproducible method. It can be used to extract water from unsaturated soil and saturated regions, while the extraction solution can be used to measure solutes in the solution, such as nutrients, dissolved organic nutrients, or pollutants; It can also be used to extract root exudates;

auxiliary equipmentEV-101Vacuum preparer

MicroRhizonandRhizonWhen collecting a large number of samples, the pore water collector usesEV-101The vacuum preparer can prepare a large number of vacuum sample tubes in a short period of time.EV-101The vacuum preparer fills helium gas by vacuuming, and then continues to evacuate. The entire preparation process is achieved by piercing the gas cylinder diaphragm with a needle. Can accommodate11The turntable of a sample tube (bottle) can easily achieve batch preparation.

Field rhizosphere secretion sampling system

The field rhizosphere secretion sampling system consists of a dedicated syringe, nutrient solution storage bottle, extension tube, and rhizosphere collection container,Rhizon MOMComposed of a type of rhizosphere secretion sampler.

main features

• In situ extraction of rhizosphere secretions in the field;

• High cost-effectiveness, suitable for a large number of settings;

• Based on the principle of capillary siphon, nutrient solution will be automatically replenished through an extension tube.

Parameters

1. Special syringe volume:20ml；

2. Root collection device volume:200ml；

3. Extension tube model:YCG-60；

4. Capacity of nutrient solution storage bottle:1L；

5. RhizonRoot exudates sampler model:MOM；

6. Extraction section: Long10cm, aperture0.2μm.

Root Box Customization and Application

Root boxes can be customized according to user needs, including specifications, sizes, materials, and matching rhizosphere pore water samplers. Tailored to your experimental design, we eliminate any worries for you.

Connect a rhizosphere pore water sampler to the root box to collect rhizosphere water at different soil depths.

Plant the plant in a root box and tilt it to observe the distribution of the root system on a transparent surface.