BD FACSCelesta Flow cytometer is an optimized multi-color flow cytometer that allows researchers to experienceBDThe tremendous innovation brought by biological science in instruments and reagents. The optical system and reagents of the instrument are updated synchronously,BD FACSCelestaEquipped with multiple activators and detectors, corresponding to brighter new dyes, it can provide you with a deeper and more favorable field of view in cell analysis.

BDThe multi laser flow cytometer is well-known for its high sensitivity and performance. stayBD FACSCelestaIn the device, its optical and electronic systemsLaser, filter, detector, optical path, and signal processing technology have all been optimized to maximize their performanceBD Horizon Brilliant The function of dyes.

BD FACSCelestaUsers can choose from four instrument configurations. All configurations include blue laser（488-nm）And purple laser（405-nm）According to your experimental needs, an additional red laser can be installed（640-nm）UV laser（355-nm）Or it could be a yellow green laser（561-nm）. By analyzing a single sample up to14 The detection of individual parameters can maximize your ability to discover in scientific experiments.

The laser combination combines new and existing technologies in a compact structure to achieve optical stability and reliable performance. The binary mirror and bandpass filter are placed in a compact polygon, and the emitted light from each laser reaches the correct detector. Detector (photomultiplier tube)PMT）Then it is compactly placed inside the filter component.

BD FACSCelestaLiquid flow system——The design of sample lines, flow trucks, sample pumps, sheath liquid tanks, and waste liquid tanks is more convenient and reliable. The latest designed digital operation panel can monitor the operation status of the flow cytometer, make adjustments at any time, and display simple indication operation modes.

Application areas:

Immunological research

BD FACSCelesta Capable of quickly and accurately analyzing various types of cells, including lymphoid tissue cells (thymus, spleen, and lymph nodes), digestive tissue cells, and blood cells.

The use of direct labeled fluorescent antibodies can detect cell surface antigens and intracellular antigens, and multi-color flow cytometry allows researchers to detect specific target proteins expressed by cells at different growth and differentiation levels. The characteristic of multi parameter measurement at the single-cell level using multi-color flow cytometry makes it important for immunological research at the gene, protein, and single-cell levels. The solutions in cell recognition, transcription factor expression, cytokine release, and measurement have been reflectedBD The commitment to high quality and consistency is also a necessary characteristic in advanced research.

Illuminate rare cells and low-density antigens

For rare cells or cells with fewer surface receptors, it is difficult to determine using traditional reagents. Separating cells with weak signals from a sample requires reagents with high fluorescence intensity.

BD Horizon BrilliantIt can make cells with weaker signals have better properties than traditional organic fluorescent dyes or phycobiliproteins（PE、APC）Stronger fluorescence signal. Optimizing these high brightness dyes can makeBD FACSCelestaDifferentiate cell groups using a wider receptor density than before.

Cell Biology Research

Flow cytometry can provide a large amount of data for cell biologists working in various fields, from intermolecular interactions to systems biology, from drug (metabolism) dynamics to tumor biology, from cell signaling to marine biology to biophysics, and more. The use of flow cytometry has enhanced proteomics research, improved the use of biomarkers in drug development, and developed high-throughput cell-based drug screening studies.

Researchers can detect the physical parameters of cells in suspension, such as cell shape, size, and internal complexity, by detecting and analyzing light scattering data; By increasing the number of fluorescent markers, proteins expressed or secreted by cells can be detected, thereby understanding the phenotype, function, and status of cells; The determination of cell function through flow cytometry can elucidate the types of samples, such as whole blood, cell lines, and yeast.BDFACSCelesta Equipped with three lasers and up to14 A detector that allows you to simultaneously measure multiple experiments on the same sample.

Single cell level study of complex taxa

One of the main advantages of flow cytometry is that it can be used to study complex taxa. Immunoblotting reaction, immunoprecipitation reaction, andPCRTechnologies rely on the dissolution of the overall sample, providing useful data about the overall sample, but it is difficult to make comparisons between different cell subpopulations; However, other techniques for detecting individual cells, such as microscopes, cannot perform quantitative analysis on samples. Flow cytometry can perform qualitative and quantitative analysis on a large number of individual cells, while also identifying subpopulations of cells with different characteristics.