1、 Product Introduction

The all-weather vegetation fluorescence observation system isEvergreen Instrument has independently developed and designed a vegetation fluorescence observation device with high resolution, high sensitivity, and 16 channels.This device uses a high-resolution and sensitive spectrometer as the main sensor, which can automatically observe the downward irradiance and upward apparent radiance of vegetation canopy in the field, and calculate the vegetation sunlight induced chlorophyll fluorescence in real time（solar-induced Fluorescence，SIF）This device can be used in the wild for a long time, 24/7. Its customized bracket can adjust the distance between the measurement sensor and the sample according to experimental needs. The device is highly modular and can be customized according to customer needs.

2、 Working principle

The 8-channel all-weather vegetation fluorescence observation system is based on spectral observations of O2-A and O2-B dark lines near 760nm and 687nm, and extracts daylight induced chlorophyll fluorescence through algorithms such as 3FLD and SFM.

The core function of this device is to measure the incident solar irradiance and the apparent radiance of vegetation canopy in a quasi synchronous manner. The incident solar irradiance is obtained by observing upwards through a fiber optic cable with a cosine corrector, and the apparent radiance of the vegetation canopy upwards is obtained by observing the canopy through another fiber optic cable, which can be fully automatically and continuously observedSix target objects, and then the data is transmitted to the host through optical fiber for computation.

The spectrometer of this device is placed in a constant temperature box. The constant temperature box adopts semiconductor technologyTEC refrigeration allows the spectrometer to operate at a constant temperature of 20 to 40 ℃± 1 ℃ (the temperature can be freely set, but the ambient temperature cannot be higher than 50 ℃ or lower than 20 ℃). Real time monitoring and storage of temperature and humidity values of the constant temperature box, as well as the ambient temperature inside the chassis. When there is an abnormal temperature, emergency measures will be automatically taken, such as alarming, shutting down the spectrometer, or even the entire system.

This equipment is equipped with fully automatic rain and dust protection devices, which can protect the cosine corrector from rain and dust invasion, and maximize the safety and reliability of the collected data of the equipment

Structural diagram

Equipment diagram

Technical specification requirements:

1. Main purpose:

Used to measure vegetation canopy reflectance and real-time solar induced chlorophyll fluorescence values

Configuration List:

Spectral system, data acquisition head, constant temperature system, data processing

Product advantages:

Automatically and continuously measure the downward irradiance and upward radiance of vegetation canopy.Automatic and rapid optimization of integration time, high signal-to-noise ratio of observation data.Real time data monitoring, automatic storage, remote transmission, and easy operation.The software can adjust the corresponding parameters according to the user's experimental plan.The instrument is compatible with solar power supply.The system can prevent dust, monitor instrument operation in real-time, and automatically cut off power protection under extreme conditions.

3. Detailed technical requirements:

1) Spectral system

It has stable signal-to-noise ratio, good spectral reproducibility, high spectral resolution, and stable system performance.

*1.1 Spectrometer (configuration can be changed according to user needs to meet better user requirements)

1.1.1 Spectrometer Range: 640-800 nm (25um slit)

1.1.2 Spectral resolution: 0.39 nm

1.1.3 Spectral sampling interval: 0.15 nm

1.1.4 Signal to Noise Ratio: 1000:1

1.1.5 CCD format: 1024 * 58

1.1.6 Pixel Array CCD

1.1.7 * High sensitivity: 200 * 103

1.1.8 Dark noise: ≤ 3RMS

1.1.9 Spectral response: Quantum efficiency * high at 780 nm (70%), quantum efficiency * high at 680 nm (50%)

*1.2 Spectrometer (configuration can be changed according to user needs to meet better user requirements)

1.2.1 Spectrometer Range: 350-1100 nm (25 slits)

1.2.2 Spectral resolution: 1.3nm, spectral sampling interval of 0.15nm, signal-to-noise ratio of 250:1

1.2.3 CCD format: 2048 pixel line array CCD

1.2.4 Dark noise: less than 12RMS

1.2.5 Spectral response: Quantum efficiency * high at 350 nm (70%), quantum efficiency * high at 680 nm (35%)

*1.3 16 channel spectroscopic system

1.3.1 Spectral range: 350-2000 nm

1.3.2 Input channels: 2 channels

1.3.3 Output channels: ≤ 16 channels

1.3.4 Measurement repeatability: 99%

1.3.5 Vegetation Fluorescence Special Software Control Multi channel Spectral System Automatic Switching and Measurement

1.3.6 Real time monitoring function: 24/7 automatic measurement of no less than 6 target areas

2) Data collection head

Data collection function: automatically optimize integration time to ensure signal-to-noise ratio of observation data; The cosine probe has dust-proof and rainproof functions; Protect the cosine corrector from rainwater and dust, and ensure the safety and reliability of the collected data of the equipment as much as possible.

2.1 High throughput fiber optic data acquisition head

2.1.1 Optional lengths: 2 meters, 5 meters, 10 meters, 20 meters (customizable)

*2.2 Rainproof device for collection head

2.2.1 Automatically block the collection head when encountering rainwater.

2.2.2 Dust cover

3) Constant temperature system

Constant temperature system function: Real time monitoring function:24/7 automatic measurement of target objects, achieving monitoring of system temperature and humidity status information.

*3.1 Thermostatic box

3.1.1 Environmental temperature: 20 ℃ -50 ℃

3.1.2 Constant temperature: 20 ℃ -40 ℃ (± 1 ℃)

3.1.3 Temperature can be freely set

3.1.4 Semiconductor TEC Refrigeration

3.1.5 Real time monitoring and storage of temperature and humidity values in the constant temperature box

3.1.6 Real time monitoring and storage of environmental temperature inside the chassis

3.1.7 Abnormal warning: When the temperature is abnormal, emergency measures will be automatically taken.

3.1.8 Warning processing: Alarm - turn off spectrometer - turn off system

4) Data processing

*4.1 Hardware of Data Processing System

4.1.1 Intel processors

4.1.2 NM70 high-speed chipset

4.1.3 Panel type: Industrial control standard screen TFT

4.1.4 Backlight type: LED

4.1.5 Brightness: 400cd/m2

4.1.6 Linear error<1.5%

4.2 Data Processing Algorithms

4.2.1 Real time calculation of SIF

4.2.2 Simultaneously has two algorithms: 3FLD SFM

*4.2.3 Simultaneously display vegetation parameters such as original spectral map, reflectance, land cover, illumination, real-time fluorescence value map, etc

4.3 Data processing function

4.3.1 Radiation correction module

4.3.2 Spectral correction module

4.3.3 Dark current correction function

4.3.4 Real time automatic optimization of integration time function

4.3.5 Measurement start and end time, adjustable measurement interval time

*4.3.6 Automatically start and stop measurement based on solar altitude angle

4.4 Other functions of data processing

4.4.1 Automatic Data Storage

4.4.2 Remote real-time data browsing

4.4.3 Data anomaly monitoring and alarm

*4.4.4 Multiple spectrograms can be displayed simultaneously, including the original spectrum, reflectance, and fluorescence values
4.4.5 Users can modify software parameters (integration time, testing time, etc.) based on on-site conditions

5. Provide customized services

Instrument functions can be added or spectrometer configurations can be changed according to user experimental requirements in the later stage. Software parameters can be modified according to on-site conditions, and the modifications will be completed and delivered to the user's site within one month. Data analysis technical support will be provided in the later stage.

Standardization proposalConfiguration List